ChIP-seq（Chromatin immunoprecipitation followed by sequencing）使用抗体富集的方法在基因组范围内检测多种蛋白（例如转录因子CTCF）和其他染色质相关蛋白（例如H3K4me3）与DNA的相互作用。ChIP-seq用于研究表观基因组如何促进细胞生长，癌症和其他疾病发生，已经成为确定不同组织、细胞的组蛋白修饰谱的金标准。
送样指南
IP后DNA：≥10 ng
细胞：5x108
组织：1 g
结果展示
经典案例
1. Collins JM, Huo Z, Wang D. ESR1 ChIP-Seq Identifies Distinct Ligand-Free ESR1 Genomic Binding Sites in Human Hepatocytes and Liver Tissue. Int J Mol Sci. 2021 Feb 2;22(3):1461
2. Namani A, Liu K, Wang S, Zhou X, Liao Y, Wang H, Wang XJ, Tang X. Genome-wide global identification of NRF2 binding sites in A549 non-small cell lung cancer cells by ChIP-Seq reveals NRF2 regulation of genes involved in focal adhesion pathways. Aging (Albany NY). 2019 Dec 28;11(24):12600-12623
3. Karolak JA, Gambin T, Szafranski P, Stankiewicz P. Potential interactions between the TBX4-FGF10 and SHH-FOXF1 signaling during human lung development revealed using ChIP-seq. Respir Res. 2021 Jan 21;22(1):26